NEW YORKAdding an antisense agent (oblimersen
sodium, Genasense) to standard dacarbazine (DTIC) may significantly improve
overall survival with only a slight increase in adverse effects, compared with
DTIC alone, according to initial results from the largest-ever phase III trial
in advanced metastatic melanoma. "We may have actually made a difference in
overall survival in patients with metastatic melanoma," said researcher Anna C.
Pavlick, DO, assistant professor of oncology, New York University School of
The antisense/DTIC combination increased response rate and
progression-free survival vs standard DTIC, data from the multicenter,
randomized, double-blind trial show. Overall survival, in the subset of
patients who have been followed for at least 12 months, was increased by 25%
(see Figure), "although further follow-up is needed . . . since these are very
preliminary data," Dr. Pavlick said at the Chemotherapy Foundation Symposium
The antisense agent targets bcl-2, a protein that is
overexpressed in melanoma and other cancer types. Researchers believe that
pretreating cancer patients with the agent may potentiate the cytotoxic effects
of chemotherapy. "This trial clearly gives us proof of concept that Genasense
can chemosensitize normally resistant tumors," Dr. Pavlick said.
The phase III trial enrolled 771 patients at 139 sites in 9
countries. Patients were randomized to receive up to eight cycles of DTIC alone
at 1,000 mg/m2 on day 1, or Genasense 7 mg/kg for 5 days followed by
infusion of DTIC 1,000 mg/m2 on day 6. Cycles were repeated every 21
days. Responders were allowed to receive another eight cycles of treatment in a
continuation protocol. Responses were evaluated every two cycles.
The median age of the predominantly Caucasian patient
population was approximately 60 years. However, some 40% of patients were over
65, a "geriatric population normally excluded from clinical trials that was
able to tolerate this therapy without significant toxicity," Dr. Pavlick noted.
Mean time from melanoma diagnosis to metastatic disease was
2.5 years. Less than 10% of patients had locally advanced unresectable disease;
the rest had metastatic melanoma. Approximately 40% of patients had received
prior immunotherapy (either high-dose interferon as an adjuvant or vaccine
Nearly 50 patients in the Genasense/DTIC arm have gone
beyond the initial eight cycles of treatment and into the eight-cycle
continuation phase, data show. "In fact, I had one patient who received those
16 cycles and is considered a complete responder, and continues to be in
complete response now approximately 2 years out," Dr. Pavlick said.
As of this report, overall survival in the entire cohort of
771 patients is not significantly different between the Genasense/DTIC and DTIC-only
arms (estimated median survival of 9.1 and 7.9 months, respectively, P =
.184). "Both groups did better than we had anticipated," Dr. Pavlick said.
"Patients on the DTIC arm were expected to survive 6 months, when they, in
fact, survived 7.9 months. The combination arm was predicted to survive 8
months, and, in fact, it was 9.1 months."
A surge of enrollment near the end of the trial meant that
the overall survival curve is "not mature," Dr. Pavlick noted, with a mean
follow-up of only 7 months. However, looking only at the 480 patients with
follow-up of 12 months or longer, there is a significant difference in overall
survival favoring the Genasense/DTIC arm (median survival of 10.1 vs 8.1
months, P = .035) (see Figure on page 2).
Estimated median progression-free survival for all 771
patients is 74 days for Genasense/DTIC vs 49 days for DTIC alone (P =
.0003). Likewise, a significant difference in time to progression was seen.
Normal LDH was found to be the best prognostic indicator of
progression-free survival, Dr. Pavlick said. Other prognostic indicators
included no liver involvement, treatment with the antisense agent, female sex,
and no prior immunotherapy. Age was not a significant prognostic indicator.
Overall tumor response was 11.7% (45 patients) in the
Genasense/DTIC arm vs 6.8% (26 patients) in the DTIC-only arm (P =
.0185); this included 5 complete responses in the combination arm and 2 in the
DTIC arm. Counting patients with stable disease (about 30% in either arm), the
total antitumor response rate was 41.7% in the combination arm and 34.3% for
DTIC alone (P = .034).
Adding the antisense agent did not lead to a reduction in
the chemotherapy dose. Cumulative DTIC dose was similar in the combination and
DTIC-only arms (mean of 3,418 mg/m2 and 3,372 mg/m2,
Adverse event rates in the combination arm were "slightly
higher" than in the DTIC-alone arm, Dr. Pavlick said: "It appears that the
antisense agent may augment the expected side effects of the chemotherapy,
namely neutropenia and thrombocytopenia, and maybe, to some extent, nausea,
headache, and anorexia. Fever was also slightly more common in the antisense
arm." The only serious adverse event seen in more than 5% of patients, however,
was fever (5.9% vs 3.1% with DTIC-alone). "That is due to the fact that fever
is attributed as a side effect of Genasense therapy," Dr. Pavlick said, "but .
. . there is not a high percentage of serious adverse events, and patients did
not come off study due to toxicity."