LOS ANGELESRadioimmunotherapy (RIT) with a radiolabeled monoclonal antibody 131I-8H9, delivered intrathecally, appears to have clinical utility when added to salvage therapy for patients with metastatic cancer to the central nervous system (CNS), according to a phase I study reported at the 2007 American Association of Cancer Research annual meeting (LB-4).
Disseminated malignant primary brain tumors and other cancers that metastasize through the cerebrospinal fluid (CSF) are challenging to treat. Antibody-based targeted therapies given through the CSF compartment have real therapeutic potential with regard to tumors that metastasize to the leptomeninges (LM), said principal investigator Kim Kramer, MD, of Memorial Sloan-Kettering Cancer Center, who presented the findings at a late-breaking trials session.
Monoclonal antibody 8H9 is a murine IgG1 antibody reactive with a wide spectrum of human solid tumors. The compound 131I-8H9, which is administered through a ventricular access device, has favorable pharmacokinetics in nonhuman primates and poses minimal toxicity.
The study involved 19 patients, aged 2 to 51 years, with refractory or recurrent CNS or LM malignancies that were shown to be 8H9 reactive. Multiple parenchymal lesions were observed in 7 patients and LM disease in 9. Tumors included neuroblastoma (n = 10), melanoma (n = 2), medulloblastoma (n = 3), pineoblastoma (n = 1), and ependymoma (n = 3).
Patients had baseline MRI of the brain and spine, and CSF cytology. Patients received a dosimetry dose of 2 mCi of intrathecal 131I-8H9, after which CSF and blood were sampled serially for pharmacokinetics, and nuclear scans were performed at 24 hours to evaluate drug localization. Therapeutic doses were 10, 20, 30, 40, and 50 mCi, with three to six patients per dose level. At 1 month post-injection, more MRI studies were done, and if no progressive disease or grade 3-4 toxicity was noted, the treatment was repeated.
The 19 patients received 56 injections: 16 patients received at least one full cycle (dosimetry and therapy injections), and 10 of the 16 received two full cycles; 3 received only the dosimetry dose. Mean dose to the CSF was 35.4 cGy/mCi, with 50% of injections being 20 to 50 cGy/mCi. Total CSF dose was 314 to 2,917 cGy. In contrast, the mean blood dose was 2.34 cGy/mCi, with an average CSF:blood ratio of 15:1 in all patients."So far, there has been a low toxicity profile, with no allergic, cardiovascular, or pulmonary toxicities," Dr. Kramer reported. "Transient grade 1-2 nausea and vomiting or fever was common in the immediate post-injection period, as was a transient rise in liver enzymes. Thus far, the maximum tolerated dose has not been reached, at 50 mCi."