Rituximab (Rituxan) is a chimeric monoclonal
antibody binding to CD20. A multicenter trial in relapsed low-grade
lymphoma (375 mg/m²/wk × 4) produced a response rate of
48%. However, patients with small lymphocytic lymphoma (SLL), the
tissue equivalent of CLL, had significantly lower response rates
(13%) and lower serum levels of rituximab. Reduced response rates in
SLL could be related to lower surface expression of CD20 antigen
and/or higher circulating B-cell counts. Both factors could also
negatively affect response rates in CLL.
In an attempt to maximize the activity of rituximab in CLL, we
conducted a phase I/II dose-escalating study. All patients received a
first dose of 375 mg/m² to minimize infusion-related side
effects. Subsequent weekly doses (three) were given at an increased
dose level (fixed for the three doses).
To date, 50 patients have been treated and analyzed. Doses ranged
from 500 to 2,250 mg/m². Of the 50 patients, 40 patients had CLL
and 10 had related disorders, including mantle cell leukemia (MCL; N
= 4), prolymphocytic leukemia (PLL; N = 2), and marginal zone
leukemia (MZL; N = 4). Median age was 66 years (range, 44 to 87
years). The majority (80%) of patients had Rai stage 3-4 disease.
Median white blood cell count was 43× 109/L (range, 1
to 334 × 109/L); hemoglobin, 10.7 g/dL (7.4 to 14.7
g/dL); platelet count, 72 × 109/L (9 to 202 × 109/L).
Median beta-2-microglobulin was 4.3 mg/L (range, 2.2 to 13 mg/L).
The median number of prior therapies was two (range, zero to six).
All patients with CLL had received prior therapy, and 53% were
refractory to fludarabine (Fludara).
With the first dose (375 mg/m²), side effects, mainly fever and
chills, were seen in all but two patients (94%). Six patients
experienced grade 3-4 toxicity, including fever, chills, and dyspnea;
five had hypotension, and one had hypertension. Five of these
patients with severe toxicity had other diagnoses (with higher
surface CD20 expression) and three had high white blood cell counts
(229,000, 200,000 and 184,000/µL, respectively).
Almost no toxicity was seen on subsequent escalated doses until the
dose reached 2,250 mg/m². No grade 3-4 toxicity was noted, but
67% (8 of 12) of patients had fever, chills, nausea, and fatigue.
The overall response rate was 40%, with 36% of patients with CLL
responding. Analysis of response by dose indicated that the lower
doses (500 to 825 mg/m²) had a response rate of 23% (6/26),
doses between 1,000 and 1,500 mg/m² had a 44% (4/9) response
rate, and doses of 2,250 mg/m², an 80% (8/10) response rate.
Analysis of response by fludarabine status revealed the response rate
in previously sensitive patients was 56% (9/16) vs 20% (4/20) in
CONCLUSION: A correlation between rituximab dose and response is
evident, and this antibody has significant activity in the treatment